How Active Honey is Tested – Understanding Bioactivity in Medicinal Honey
Honey
Matt Fewster
Matt Fewster
Sep 3, 2025
6 min read
How Active Honey is Tested – Understanding Bioactivity in Medicinal Honey
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Why Testing Matters in Medicinal Honey

For businesses sourcing medicinal-grade honey, bioactivity testing is crucial. Consumers are no longer satisfied with vague claims, scientific validation is what builds trust, ensures compliance, and differentiates a premium honey product from ordinary honey. Testing methods provide clear, measurable data on a honey’s antibacterial strength, ensuring that buyers receive a product with proven medicinal benefits. Among the widely used methods, the Well-Diffusion Phenol Equivalent (WDPE) test remains the gold standard for measuring antimicrobial activity. Here’s how it works and why Western Australian active honey consistently ranks among the most potent medicinal honeys in the world.

1. The Well-Diffusion Phenol Equivalent (WDPE) Test – Measuring Total Activity (TA)

The Well-Diffusion Test is the industry-recognised method for assessing antibacterial capability in honey. It measures Total Activity (TA), which reflects the honey’s ability to inhibit bacterial growth in a controlled lab environment. How the WDPE Test Works:

  1. A diluted sample of honey is placed into a small well inside a petri dish containing a thin layer of agar infused with bacteria (e.g., Staphylococcus aureus).
  2. Over 24 hours, the honey’s antimicrobial compounds diffuse into the agar, preventing bacterial growth.
  3. The bacteria-free zone around the honey sample is measured and compared to a phenol standard (a powerful antiseptic).
  4. The result is expressed as Total Activity (TA), which represents the honey’s overall antibacterial potency.

What the TA Score Means

  • TA10+ = Moderate antimicrobial strength
  • TA20+ = Strong antimicrobial activity
  • TA30+ = Highly active honey
  • TA40+ and above = Exceptional capacity, often exceeding medical-grade honeys

Since this test measures both Peroxide Activity (PA) and Non-Peroxide Activity (NPA), it provides a more complete assessment of honey’s antibacterial power than single-factor testing.

Key takeaway: TA is the most widely accepted measure of honey’s medicinal potential, ensuring buyers receive products with scientifically verified antimicrobial strength.

2. Western Australian Active Honey vs. Manuka Honey – What the Data Shows

Manuka honey has long been recognised as a leading medicinal honey, supported by strong consumer awareness and established grading systems. However, scientific testing shows that Western Australian active honeys such as Jarrah, Marri, and Blackbutt offer distinct advantages, particularly in the area of Total Activity (TA).

Western Australian Active Honey (Jarrah, Marri, Blackbutt)

  • Measured by Total Activity (TA) – a combination of Peroxide Activity (PA) and Non-Peroxide Activity (NPA).
  • Strong Peroxide Activity (PA) delivers a stable antimicrobial effect when activated by moisture (e.g., saliva, wounds).
  • Naturally rich in antioxidants and prebiotic compounds, supporting applications in gut health and immune-focused products.
  • Proven bioactivity up to TA50+, placing it among the world’s most active natural honeys.
  • Harvested from Western Australia’s disease-free forests, free from antibiotics, artificial feeding, or chemical intervention.

Manuka Honey (New Zealand)

  • Measured by Non-Peroxide Activity (NPA), UMF™, and MGO (Methylglyoxal).
  • Antimicrobial activity is primarily linked to MGO, which develops naturally as the honey ages.
  • Manuka has strong market recognition and an established grading framework, making it a trusted choice for many businesses.

Key Takeaway:

Both Manuka and Western Australian honeys are premium, medicinal-grade products. The key difference lies in their activity profile: Manuka’s strength is linked to MGO and NPA, while Western Australian honeys consistently show high Total Activity, offering broad-spectrum, stable antimicrobial effects. For businesses, this means WA honey can complement or enhance product ranges already featuring Manuka, providing differentiation backed by science.

3. How Buyers Can Use These Insights to Make Better Decisions

For importers, retailers, and brands looking to source medicinal honey, relying on marketing claims is a high-risk approach. Instead, businesses should focus on third-party test results, transparent grading systems, and scientifically validated methodologies. Here’s how to apply this knowledge when sourcing honey:

1. Always Verify Total Activity (TA) Levels

  • Request independent lab results showing WDPE test data for each batch.
  • Ensure TA scores align with medicinal use - TA30+ is the benchmark for high bioactivity.

2. Understand the Stability of Bioactive Compounds

  • Western Australian honey provides stable antimicrobial effects when exposed to moisture, making it ideal for functional foods, supplements, and topical applications.
  • Manuka’s MGO-based activity can degrade over time, meaning storage conditions impact its effectiveness.

3. Choose Honey That Aligns with Your Market and Product Needs

  • Functional food & supplement brands should prioritise stable bioactivity and proven prebiotic effects (e.g., Western Australian Jarrah).
  • Medical and pharmaceutical industries should look for high TA scores and independent verification of antimicrobial strength.
  • Retailers and wellness brands should ensure their honey is sustainably sourced, free from artificial treatments, and compliant with export regulations.
The Future of Medicinal Honey Testing

As consumer demand for scientifically validated honey grows, businesses that prioritise test-backed, high-TA products will hold a significant advantage in the global market.

Western Australian honey’s combination of high TA, strong PA/NPA balance, and stable bioactivity makes it a superior choice for medicinal and functional applications. With ongoing research and improved honey grading systems, buyers now have access to better data, clearer sourcing standards, and more reliable supply chains than ever before.

For businesses looking to source premium active honey with proven bioactivity, the next step is simple:

Demand transparency, request independent test results, and choose honey that offers verified medicinal benefits, backed by science, not just marketing.

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